redd1  (Santa Cruz Biotechnology)

Journal: American Journal of Translational Research

Article Title: A novel signature based on twelve programmed cell death patterns to predict the prognosis of lung adenocarcinoma

doi: 10.62347/UAMN8558

Figure Lengend Snippet: DDIT4 in pan-cancers. A. Histogram of DDIT4 expression levels in BEAS-2B and A549 cell lines. Data represent the means ± SD of 3 independent experiments and t test was used to analyze the difference. Variables are presented as mean ± SD. B. Forest plot of cancer types whose prognosis was significantly correlated with DDIT4. C. Differential analysis of DDIT4 expression in pan-cancers obtained from the TIMER 2.0 database. *: P < 0.05, **: P < 0.01, ***: P < 0.001. Variables are presented as mean ± SD.

Article Snippet: Antibodies were purchased from manufacturers: DDIT4 (Santa Cruz, USA, sc-271158), β-Actin (CST, USA, #3700).

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: A novel signature based on twelve programmed cell death patterns to predict the prognosis of lung adenocarcinoma

doi: 10.62347/UAMN8558

Figure Lengend Snippet: DDIT4 knock-down retarded the proliferation of LUAD cells in vitro. (A) qPCR and WB showed the efficiency of siRNAs. Data represent the means ± SD of 3 independent experiments and t test was used to analyze the difference. (B, C) Colony formation assays and growth curves (days 1-4) represent the proliferation of A549/PC9 cells infected with si-NC or DDIT4-si1/2. Representative images of the crystal violet staining of cells in 6-well plates were shown. Data represent the means ± SD of 3 independent experiments and t test was used to analyze the difference. (D, E) DDIT4 knockdown resulted in increased apoptosis in LUAD cells. Representative FACS images were shown. Data represent the means ± SD of 3 independent experiments and t test was used to analyze the difference (D), and representative in situ fluorescence images (20× magnification) were also shown (E). Scale bars = 100 μm. *: P < 0.05, **: P < 0.01, ***: P < 0.001. Variables are presented as mean ± SD.

Article Snippet: Antibodies were purchased from manufacturers: DDIT4 (Santa Cruz, USA, sc-271158), β-Actin (CST, USA, #3700).

Techniques: Knockdown, In Vitro, Infection, Staining, In Situ, Fluorescence